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KMID : 0881720210360010024
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Journal of Food Hygiene and Safety
2021 Volume.36 No. 1 p.24 ~ p.33
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Determination of Mycotoxins in Agricultural Products Used for Food and Medicine Using Liquid Chromatography Triple Quadrupole Mass Spectrometry and Their Risk Assessment
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Choi Su-Jeong
Ko Suk-Kyung
Park Young-Ae
Jung Sam-Ju
Choi Eun-Jung
Kim Hee-Sun
Kim Eun-Jung
Hwang In-Sook
Shin Gi-Young
Yu In-Sil
Shin Yong-Seung
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Abstract
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For this study, we surveyed concentrations of 8 mycotoxins (aflatoxin B1, B2, G1, G2, ochratoxin A, fumonisin B1, B2 and zearalenone) in agricultural products used for food and medicine by liquid chromatographytandem mass spectrometry and conducted a risk assessment. Samples were collected at the Yangnyeong Market in Seoul, Korea, between January and November 2019. Mycotoxins were extracted from these samples by adding 0.1% formic acid in 50% acetonitrile and cleaned up by using an ISOLUTE Myco cartridge. The method was validated by assessing its matrix effects, linearity, limit of detection (LOD), limit of quantification (LOQ), recovery and precision using four representative matrices. Matrix-matched standard calibration was used for quantification and the calibration curves of all analytes showed good linearity (r2>0.9999). LODs and LOQs were in the range of 0.02-0.11 μg/kg and 0.06-0.26 μg/kg, respectively. Sample recoveries were from 81.2 to 118.7% and relative standard deviations lower than 8.90%. The method developed in this study was applied to analyze a total of 187 samples, and aflatoxin B1 was detected at the range of 1.18-7.29 μg/kg (below the maximum allowable limit set by the Ministry of Food and Drug Safety, MFDS), whereas aflatoxin B2, G1 and G2 were not detected. Mycotoxins that are not regulated presently in Korea were also detected: fumonisin (0.84-14.25 μg/kg), ochratoxin A (0.76-17.42 μg/kg), and zearalenone (1.73- 15.96 μg/kg). Risk assessment was evaluated by using estimated daily intake (EDI) and specific guideline values.
These results indicate that the overall exposure level of Koreans to mycotoxins due to the intake of agricultural products used for food and medicine is unlikely to be a major risk factor for their health.
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KEYWORD
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Agricultural products used for food and medicine, Mycotoxins, Risk assessment
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